The ease of reversed phase meets the power of normal phase. With the ACQUITY UPC2 System, the orthogonal capability of normal-phase LC has been elevated to a mainstream technique: Convergence Chromatography. ▪ The chromatographic principles and selectivity of normal-phase LC ▪ The ease-of-use and method development simplicity of reversed-phase LC ▪ The use of gradients across the widest polarity range ▪ Chiral and achiral separations in one system, with unequaled speed and confidence Features and Benefits Bringing ease-of-use to normal-phase LC By providing the ability to precisely vary mobile phase strength, pressure, and temperature, the Waters ACQUITY UPC2 System gives scientists the power to better control the retention of analytes for separating, detecting, and quantifying structural analogs, isomers, and enantiomeric and diasteriomeric mixtures. The world’s first convergence chromatography system Unique in its capabilities, the pioneering ACQUITY UPC2 System delivers what the rest of the industry never could – a system, based on the principles of normal-phase LC, with the ease-of-use of reversed-phase LC, and purposefully built for analytical performance, placing convergence chromatography atop the list of separations tools in the kit of all analytical chemists. Retention mechanisms and orthogonality to RPLC Harnessing what’s now known as convergence chromatography, an evolution of the traditional SFC technique, the ACQUITY UPC2 System has the power to create selective separations of structurally similar chiral and achiral compounds. The miscibility of CO2with a wide range of polar and non-polar organic solvents has made the liquid CO2-based mobile phase versatile enough to separate a much wider range of compounds than reversed-phase LC, especially for mixtures containing polar compounds. Not only can CO2-based solvents be used with both polar and non-polar stationary phases, but chromatography can be influenced by modulating solvent gradients with a much wider choice of columns using the same mass spectrometry-compatible co-solvents. Being orthogonal to reversed-phase LC, UPC2 separations often show an inverse order of elution among groups of analytes. In combination with various detection techniques, this orthogonality is valuable for confirming analyte identity in complex matrices.