Workflow
The microbiology workflow involves sample collection, isolation and cultivation of microorganisms, identification, quantification, and proper storage or disposal, ensuring accurate analysis and safe handling of microbial specimens.
Sample Collection
Using sterile techniques to obtain a representative sample, properly labeling it, and transporting it to the laboratory under conditions that preserve its integrity for accurate microbial analysis.
Isolation & Cultivation
Selecting a pure sample from a mixed culture, using selective media to grow specific microorganisms, and providing optimal conditions for their growth and development for further identification and study.
Identification
A combination of morphological, biochemical, and molecular techniques to accurately determine the species or strain of microorganisms present in a sample.
Quantification
Measuring the number of viable microorganisms in a sample using methods such as colony counting, turbidity measurement, or molecular assays to determine microbial concentration.
Storage & Decontamination
Properly preserving microbial cultures under suitable conditions to maintain viability while ensuring the safe disposal or inactivation of hazardous materials to prevent contamination and spread of microorganisms.
Workflow
The microbiology workflow involves sample collection, isolation and cultivation of microorganisms, identification, quantification, and proper storage or disposal, ensuring accurate analysis and safe handling of microbial specimens.
Nucleic Acid Extraction
Nucleic acid extraction is used in molecular biochemical tests and diagnosis, and is the first step of cloning, transformation, enzyme digestion, in vitro transcription, amplification, and sequencing.
Quantification
Checking of the purity and quantity of the Nucleic acid prior to PCR mix preparation step.
Polymerase Chain Reaction
Polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample allowing scientists to take a very small sample of DNA and amplify it (or a part of it) to a large enough amount to study in detail.
Gel Electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge.
Gel Documentation
For imaging and documentation of nucleic acid and protein suspended within polyacrylamide or agarose gels.
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